By Teresa Rocha-Santos, Armando C. Duarte
Seas and oceans supply quite a lot of temperature, strain, gentle and chemical conditions thus permitting a large variety of marine organisms from shallow coastal waters to the deep ocean. those assets can be utilized to procure new items and strengthen companies, and in flip support to provide solutions to the demanding situations that have an effect on our planet, together with delivering a sustainable offer of meals and effort, new business fabrics and procedures, new bioactive compounds, and new well-being remedies. Marine compounds were pointed out as having antibacterial, anticoagulant, antifungal, antimalarial, antiprotozoal, antituberculosis, and antiviral actions. the most important assets of those bioactive compounds are marine sponges, coelenterates, and microorganisms, by way of algae, echinoderms, tunicates, molluscs, and bryozoans.
The discovery of bioactive compounds from marine samples is a scorching subject contemplating the present need for sustainable use of marine assets. This publication is a complete evaluation of the analytical techniques employed within the discovery and characterization of bioactive compounds remoted from (all attainable) marine samples and offers destiny views of analytical methodologies. This evaluate contains an review of the sampling and instruction of extracts, the separation and isolation of bioactive compounds, their structural characterization and the applying of bioassays within the discovery of bioactive compounds.
- Comprehensive assurance of analytical ideas and applications
- Clear diagrams to thoroughly help very important topics
- Real examples of purposes of analytical strategies within the look for new bioactive compounds
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Additional info for Analysis of Marine Samples in Search of Bioactive Compounds
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TLC was used for further fractionation (silica gel).  2,2,7-trimethyl-2Hchromen-5-ol Ethyl acetate and dichloromethane Column chromatography with silica gel using a stepwise elution with mixtures of dichloromethane and methanol (between 100:0 and 0:100). TLC was used for further fractionation (silica gel).  RP-HPLC: Reverse-phase high performance liquid chromatography; TLC: Thin-layer chromatography; SPE: Solid Phase Extraction. ) Marine Organism Origin Bioactive Compounds Solvent Extraction Fractionation and Purification Reference SPONGES Xestospongia testudinaria South Pacific Halenaquinonetype polyketides Dichloromethane and dichlorometane: methanol (1:1) Silica gel column chromatography (230–400 mesh) with 20% methanol in dichloromethane as eluent.
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Analysis of Marine Samples in Search of Bioactive Compounds by Teresa Rocha-Santos, Armando C. Duarte